Bacterial Type Transformation

Impact of heat shock step on bacterial transformation efficiency

CaCl2 treatment followed by heat shock is the most common method for artificial transformation. Here, the cells were transformed using CaCl2 treatment either with heat shock (standard protocol) or without heat shock (lab protocol) to comprehend the difference in transformation efficiency. The BL21 strain of Escherichia coli (E. coli) was being susceptible using CaCl2 treatment. Some Cells were ...

Bacterial transformation reactions.

In reviewing previously the chemical nature and biologic specificity of the substance inducing transformation of pneumococcal types (31), McCarty suggested that the principles involved and the results observed in the study of this reaction have implications beyond those referable to the biology of pneumococcus. The correctness of this point of view has been borne out by the investigations of th...

The bacterial transformation of abietic acid.

An Alcaligenes species, which was isolated from soil, can utilize abietic acid as its sole carbon source. During growth, the bacterium transforms abietic acid into 5alpha-hydroxyabietic acid (I, R=OH), a product considered to be 7beta-hydroxy-13-isopropyl-8xi-podocarp-13-en-15-oic acid (II, R=H) and a compound, C(20)H(28)O(3), which is believed to be an epoxy-gamma-lactone.

Ethanol mediated enhancement in bacterial transformation

In molecular biology, transformation using E. coli as a host plays a key role in synthesizing gene libraries. The present study demonstrated a new ethanol-based method for transformation of plasmid DNA to E. coli. Ethanol at 10% concentration (v/v) showed best results. Further, as compared with traditional CaCl2 method, the transformation rate, using protocol outlined in this study, was very hi...

Models for Dna Mediated Bacterial Transformation